5.14 Humulin

• The bacterial cell is transformed by the recombinant DNA
• Human DNA for the insulin gene is combined with the plasmid DNA
• A large population of the bacteria is injected into a fermenter
• Necessities
• Provides the right nutrients
• Controls the temperature
• Controls the pH level
• Controls the gases in the fermenting chamber
• Optimum temperature for bacterial growth > population increase + manifacture the protine insulin
• The insulin protein will be manufactured from the amino acid (nutrient protein) by the bacteria
• Necessary to remove the product to carry out purification by downstream processing
• This genetically engineered human insulin is called 'Humulin'