5.13 Recombinant DNA and Hosting Recombinant DNA

• Plasmid
• Found in bacterial cells
• Is a small circular piece of DNA
• Doesn't carry a lot genes
• Virus
• Contains Caspid
• protein shell
• Contains DNA/RNA
• A nucleic acid
• Has no nucleus or cytoplasm
• Human Chromosome 
• Identify the gene (Insulin- Hormone controling the blood sugar levels)
• The selected restriction enzyme cuts the DNA (Cuts the insulin gene)
• Cut the gene
• Isolate the plasmids and cut with the same enzyme
• the plasmids are now open allowing the insulin gene to be mixed
• Vector
• Transfers the gene into the plasmid
• The nucleic acid is removed from the virus to obtain the Caspid protein shell
• The virus takes the plasmids and acts as a vector for the recombiant DNA
• This helps transfer the DNA to the host
• Phage
• A type of virus
• Infects bacteria cells
• The virus is able to insert the recombinant DNA into the host cell by attaching itself to the cell membrane of the bacteria host
• the recombinant DNA and the human gene for insulin is now in the bacteria cell
• This is a combination called 'transgenic